The game follows the adventures of Mario, the iconic plumber, as he navigates the mystical realm of Glitzville. Mario's quest begins when he receives a letter from his friend, Goombella, inviting him to meet her in Glitzville. Upon arrival, Mario finds himself entangled in a mysterious plot to awaken the ancient powers of the Thousand-Year Door. Joined by a diverse cast of allies, including Goombella, Kooper, and Vivian, Mario must explore the world, battle formidable foes, and unravel the secrets behind the door.